| # | Name | POS | H | W | School | Hometown |
| 9 | Clinton Alford | QB | 6'0" | 190 | Columbia High | Lake City |
| 5 | LaJavier Baker | OL/DL | 6'0" | 324 | Columbia High | Lake City |
| 69 | Chris Boland | OL | 6'5" | 350 | Ft White | Ft White |
| 10 | Chris Bradshaw | DB/WR | 6'2" | 165 | Columbia High | Lake City |
| 12 | Jonathan Branch | DE | 6'1" | 166 | Dixie County | Old Town |
| 54 | Andrew Carter | OL/DT | 6'8" | 312 | Hamilton County | Jasper |
| 23 | William Clemons | WR/DB | 5'8" | 150 | Branford High | Branford |
| 3 | Adam Crumitie | DB | 5'11" | 175 | Columbia High | Lake City |
| 4 | George Cruz | DB | 5'9" | 190 | Booker T. Washingto | Lake City |
| 45 | Jon Dewitt | LB | 5'11" | 225 | Columbia High | Lake City |
| 8 | Chris Dickey | DB | 5'9" | 187 | Columbia High | Lake City |
| 13 | Jermaine Dye | QB/TE | 6'2" | 230 | Berea | White Springs |
| 17 | Austin Eichman | FB/LB | 6'0" | 240 | Pace High | Lake City |
| 1 | Lorne Emmanuel | DB | 5'10" | 188 | Bethel | Jasper |
| 35 | Buddy Fleming | FB/DT | 6'3" | 253 | Columbia High | Lake City |
| 32 | Davion Gainer | WR/CB | 6'0" | 167 | Columbia High | Lake City |
| 25 | Steven Gamble | DB | 6'1" | 156 | Columbia High | Lake City |
| 44 | Lorenzo George | LB | 5'10" | 250 | Dixie County | Cross City |
| 22 | Geoffery Harrell | LB | 5'7" | 215 | Columbia High | Lake City |
| 15 | Alvin Hawkins | TE/DB | 6'3" | 200 | Andrew Jackson | Lake City |
| 18 | KC Hutcherson | RB/DB | 5'9" | 212 | Hamilton | Lake City |
| 84 | Frankie Jones | WR | 5'11" | 130 | Newberry High | Newberry |
| 99 | Ronald Kilts | OL/DT | 6'3" | 360 | Terry Parker | Lake City |
| 26 | Desmond Kiser | DB | 6'0" | 160 | Hamilton High | Jasper |
| 19 | Jamel Leach | TE/DE | 6'4" | 220 | A.L Brown High | Lake City |
| 6 | Jelani Newton | WR | 5'10" | 190 | Columbia High | Lake City |
| 95 | DJ Pierce | DT | 5'11" | 245 | Columbia High | Lake City |
| 67 | John Quincy | OL/DL | 5'9" | 285 | Suwanne High | Lake City |
| 55 | Theis Rossin | LB | 6'5" | 260 | Famu | Lake City |
| 7 | Luis Santiago | RB/DB | 6'0" | 223 | Dixie County | Lake City |
| 87 | Robert Singleton | WR | 6'0" | 200 | Terry Parker | Lake City |
| 49 | Anthony Smith | OL/DT | 6'1" | 230 | Dixie County | Cross City |
| 11 | Trenton Thompson | RB/DB | 5'9" | 165 | Branford High | Branford |
| 41 | Carlos Vega | LB | 5'7" | 190 | Dixie County | Lake City |
| 2 | Andre Walters | WR/DB | 5'8" | 225 | Oak Grove High | Lake City |
| 56 | Kevin Wethy | LB | 6'0" | 190 | Union County | Lake Butler |
| 77 | Scott Wethy | DE | 6'3" | 215 | Union County | Lake Butler |
| 27 | Jai Whitfield | RB | 6'0" | 230 | Randolph High | O'Brien |
| 24 | Johnny Williams | LB/FB | 6'1" | 240 | Columbia High | Lake City |
| # | Name | POS | Hometown |
| Luis Santiago | Team Owner | Lake City | |
| Elaine Harden | Team Owner | Lake City | |
| Derek Shiver | Head Coach | Lake City |